International Journal of Recent Trends in Engineering & Research

online ISSN

Next Generation Sequencing Quality trimming

Publication Date : 01/05/2016


Author(s) :

Danamma B.Javalimath , Naveen Kumar , V.G.Shanmuga priya.


Volume/Issue :
Volume 2
,
Issue 4
(05 - 2016)



Abstract :

Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. NGS refers to high-throughput sequencing that parallelizes the sequencing process, producing millions or billions of sequences concurrently. NGS generates enormous amount of data, a single sequencing run could produce about one tear bytes (TB) of data which is difficult to maintain and the presence of poor quality or technical sequences such as adapters in NGS data can easily result in suboptimal downstream analysis so data needs to be pre-processed. Before analyzing data, quality checking and trimming of data is important. To do this Trimmomatic tool is used to check the quality of data generated from Illumina sequencing. Trimmomatic is a command prompt tool it requires more computational knowledge to perform, for a non-technician or for a biologist. To overcome this issue it is necessary to develop a GUI for a Trimmomatic tool, which helps biologist to perform quality check with less computational knowledge. A transition from a Trimmomatic command prompt tool to Graphical User Interface (GUI) is done using java where user can do quality check in minimal button clicks and time saving. Keywords-Next-generation sequencing, Adapter trimming, Data pre-processing, command line, Graphical User Interface.


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