Preliminary Phytochemical Screening, Acute Oral Toxicity and Anticonvulsant Activity of the Rhizomes of Acorus gramineus Soland.
Publication Date : 01/03/2016
The present of study is carried out to investigate the preliminary phytochemical properties, acute oral toxicity and anticonvulsant activity of the rhizomes of Acorus gramineus Soland. (A. gramineus). Standard methods were applied to screen phytochemicals from the methanol rhizomes extracts. The performance of acute oral toxicity study following Organization for Economic Cooperation and Development (OECD) 425 guidelines had been run before the anticonvulsant activity evaluation of resisting pentylenetetrazole (PTZ) induced seizure in mice was figured out. In the dose at levels of 100, 200, 300 and 400 mg/kg body weight, the treatment’s impact was estimated through an experimental mice model and compared with phenobarbital (100 mg/kg p.o.) as positive control. Histopathological analysis is also applied for verifying the direct effects of A. gramineus extract on mice’s brain. Carbohydrates, flavonoids, alkaloids, tannins, phenolic compounds, anthraquinones, steroids and terpenoids are qualified in phytochemical screening. The oral median lethal dose of the rhizome extracts was estimated as upper 5000 mg/kg body weight. In PTZ-induced seizures, the extracts significantly retarded the latency of convulsant (p < 0.05) in at dose of 300 and 400 mg/kg p.o., decreased the frequency of convulsant and increased up to 100% protection resistant to death. The histopathological analysis revealed the impacts of extract on brain tissue in the dose of 300 and 400 mg/kg p.o. The findings proved in this study suggest that the methanol extracts of A. gramineus rhizomes is dependable and high potential in anticonvulsant activity in PTZ-induced seizure in mice. Keywords: Acorus gramineus, Anticonvulsant, Phytochemical, Acute Oral Toxicity, Lethal Dose (LD50), Pentylenetetrazole, Histopathological.
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